Search Results (3 total)

Intracellular transport of large cargoes, such as organelles, vesicles, or large proteins, is a complex dynamical process that involves the interplay of adenosine triphosphate–consuming molecular motors, cytoskeleton filaments, and the viscoelastic cytoplasm. In this work we investigate the motion of pigment organelles (melanosomes) driven by myosin-V motors in Xenopus laevis melanocytes using a high-spatio-temporal resolution tracking technique. By analyzing the obtained trajectories, we show that the melanosomes mean-square displacement undergoes a transition from a subdiffusive to a superdiffusive behavior. A stochastic theoretical model, which explicitly considers the collective action of the molecular motors, is introduced to generalize the interpretation of our data. Starting from a generalized Langevin equation, we derive an analytical expression for the mean square displacement, which also takes into account the experimental noise. By fitting theoretical expressions to experimental data we were able to discriminate the exponents that characterize the passive and active contributions to the dynamics and to estimate the “global” motor forces correctly. Then, our model gives a quantitative description of active transport in living cells with a reduced number of parameters.

Biology is complex. However, it is not clear how much of this complexity must necessarily translate into complicated mathematical models of biological processes. Simple models can be appealing to physicists but are usually deceiving for biologists. Complicated models, on the other hand, depend on too many parameters whose values are frequently unknown. Therefore, complicated models, although in principle more realistic, can lead to erroneous results if they are sensitive to these unknown parameter values. Intracellular calcium signals provide an example of utmost biological importance in which the issue of “simple vs complex” can be explored. In this paper we show that simple models describing the dynamics of intracellular calcium can be directly inferred from experimental data, without no a priori information on unknown parameters. A similar approach can be followed to study other reaction-diffusion systems. In spite of their simplicity, these models can provide quantitative information on some of the processes that shape calcium signals, such as the calcium current that underlies an experimental observation. This shows that simple models of biological systems are not limited to qualitative descriptions.

We describe the status of our effort to realize a first neutrino factory and the progress made in understanding the problems associated with the collection and cooling of muons towards that end. We summarize the physics that can be done with neutrino factories as well as with intense cold beams of muons. The physics potential of muon colliders is reviewed, both as Higgs factories and compact high-energy lepton colliders. The status and time scale of our research and development effort is reviewed as well as the latest designs in cooling channels including the promise of ring coolers in achieving longitudinal and transverse cooling simultaneously. We detail the efforts being made to mount an international cooling experiment to demonstrate the ionization cooling of muons.